Basic types and principles of photometric enzyme-linked immunosorbent assay (ELISA)
From the establishment to the present thirty years, ELISA technology has become increasingly mature, the most classic method is the double antibody sandwich method established by Adans3 in 1977. In order to save time and save materials, many improved methods have been established and can be successfully used for virus detection and identification.
Solid phase enzyme immunoassays are used to determine antigens and can also be used to determine antibodies. There are three essential reagents in this assay: 1. Solid phase antigen or antibody; 2. Enzyme-labeled antigen or antibody; 3. Enzyme reaction substrate. Depending on the source of the reagent and the nature of the specimen and the specific conditions of the assay, various types of assays can be devised.
First, double antibody sandwich DAS (ELISA)
Second, indirect method (Indirect ELISA)
Third, the competition law (Competing ELISA)
Fourth, the heterogeneous animal double antibody sandwich method, also known as indirect double antibody sandwich method (InduirectDAS-ELISA) or triple antibody sandwich method (TAS-ELISA)
Five, anti-enzyme antibody method
Six, competitive inhibition method
Seven, double sandwich method
Eight, antigen direct coating method
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