Experimental principle of human pro-atrial natriuretic peptide (Pro-ANP) ELISA kit

English name: Human Pro Atrial Natriuretic Peptide, Pro-ANP ELISA

1. The level of human pro-ANP (Pro-ANP) ELISAKit in the specimen was determined by double antibody sandwich method.

2. Firstly, the microwell plate is coated with purified human pro-atrial natriuretic peptide (Pro-ANP) ELISAKit antibody to prepare a solid phase antibody.

3. Then add pro-ANP (Pro-ANP) ELISAKit to the microwells coated with mAb, and then combine with HRP-labeled Pro-ANP (Pro-ANP) ELISAKit antibody to form antibody-antigen-enzyme labeled antibody After thorough washing, the complex is added with substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

4. The color depth is positively correlated with Pro-ANP ELISAKit in the sample.

5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human Pro-ANP in the sample is calculated by a standard curve.

Objective: To determine the content of Pro-ANP ELISAKit in human serum, plasma and related liquid samples.

6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.

7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.

safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.

4 Keep Elisa kit away from children

Bring your own items:

1. 37 â„ƒ incubator

2. Standard specification microplate reader

3. Precision pipette and disposable tip

4. Distilled water

5. Disposable test tube

6. Absorbent paper

Storage conditions and validity period:

1. Kit storage :; 2-8 â„ƒ.

2. Validity: 6 months

Product Range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit

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