Experimental principle of human tumor necrosis factor soluble receptor â… kit
1. The level of soluble human tumor necrosis factor receptor â… Kit in the specimens was measured by double antibody sandwich method.
2. First coat the microplate with purified human tumor necrosis factor soluble receptor I Kit antibody to make a solid phase antibody.
3. Then add the tumor necrosis factor soluble receptor â… Kit to the monoclonal antibody-coated microwells in turn, and then combine with HRP-labeled tumor necrosis factor soluble receptor â… Kit antibody to form an antibody-antigen-enzyme labeled antibody complex, which is thoroughly washed After adding substrate TMB color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the soluble receptor of tumor necrosis factor I Kit in the sample.
5. Finally, the absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human tumor necrosis factor soluble receptor IKit in the sample was calculated from the standard curve.
Objective: To determine the content of tumor necrosis factor soluble receptor â… Kit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children's own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months Related products:
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Product Range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit
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