Beta-stimulant enzyme-linked immunoassay kit

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1. Introduction to the kit
This kit is suitable for quantitative testing of β-stimulant families contained in tissues, feed and urine samples. The kit has stable performance, convenient operation and high sensitivity.
The kit includes the following:
Chinese name
English name
specification
Antibody-coated microplate (removable)
Microtitre Plate
12 × 8 holes
Diluent / wash buffer (concentrated) (green cap)
DiLuent / Wash Buffer (Conc.)
1 × 32 mL
Enzyme marker antigen diluent (blue cap)
Conjugate Diluent
1 × 20 mL
Enzyme labeled antibody (concentrated)
Conjugate (Conc.)
1 bottle
Single-component substrate / coloring agent (brown bottle)
One Shot Substrate
1 × 15 mL
Standard solution (black cap)
Standards
6 × 2 mL
Reaction stop solution (blue cap plastic bottle)
Stop Solution
1 × 15 mL
Note: All concentrated liquids need to be used after dilution. For the dilution method, see reagent preparation and use
Lower detection limit
beta-stimulant family
Pee
organization
feed
Clenbuterol
0.1 ng / mL
0.2 ng / g
1.1 ng / g
Cabotro
1.1 ng / mL
0.6 ng / g
——
Salbutamol
0.2 ng / mL
0.3 ng / g
2.0 ng / g
Methyl clenbuterol
1.3 ng / mL
0.7 ng / g
——
Brombutrol
2.0 ng / mL
1.0 ng / g
——
Terbutaline
2.0 ng / mL
1.0 ng / g
——
Maputro
2.2 ng / mL
1.1 ng / g
——
Pibuterol
3.2 ng / mL
1.6 ng / g
——
Mottro
4.0 ng / mL
2.0 ng / g
——
Cimatro
10.0 ng / mL
5.0 ng / g
——
2. Principle of SU2148 β-Doping Kit
1. The enzyme-labeled plates that can be disassembled into strips have been coated with β-stimulant group-specific antigens.
2. The β-stimulant family in the standard solution or sample will compete with the enzyme marker for the binding site of the antibody on the enzyme plate to form an antibody / antigen complex or antibody / enzyme labeled antigen complex.
3. Wash the enzyme label plate to remove free enzyme label. Add substrate / colorant. Within a certain temperature range, the antibody / enzyme-labeled antigen complex catalyzes the color (blue) reaction of the substrate / chromogen. After the addition of acid, the reaction is terminated and the color of the reactant changes from blue to yellow.
The depth of color is inversely proportional to the content of the β-stimulant family. On a microplate reader with a wavelength of 450 nanometers (nm), the corresponding absorbance can be read.

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