The analytical centrifuge uses a specially designed rotor and optical inspection system to continuously detect the sedimentation process of the material in the centrifugal field to determine its physical properties.
The turret of the analytical ultracentrifuge is elliptical to avoid stress concentration at the holes. The rotor is connected to a high-speed drive through a flexible shaft that rotates in a freezer and vacuum chamber with 2 to 6 chambers containing centrifuge cups. The centrifuge cup is fan-shaped quartz. Can pass light up and down. The centrifuge is equipped with an optical system that monitors the settled material in the centrifuge cup by ultraviolet light absorption or refractive index change throughout the centrifugation, and a photograph of the settled material can be taken for a predetermined period of time. When analyzing the material settling condition in the centrifuge cup, the interface formed between the heavy particles and the light particles acts like a refracting lens, resulting in a "peak" on the photographic floor of the inspection system. As the sedimentation progresses, the interface advances, so the peak also moves, and the sedimentation velocity of the sample particles can be calculated from the speed of the peak movement.
Analytical ultracentrifuges can get some important information in a short time with a small amount of sample. For example, it is possible to determine the presence and approximate content of biomacromolecules; calculate the sedimentation coefficient of biomacromolecules; estimate the size of molecules in combination with interface diffusion; detect molecular heterogeneity and the proportion of components in the mixture; The relative molecular mass; it is also possible to detect conformational changes of biological macromolecules.
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