Antibiotics are substances produced by microorganisms (including bacteria, fungi, and actinomyces) that can inhibit or kill other microorganisms. In addition to antibacterial, it is also widely used in anti-tumor, anti-virus, antiprotozoa, parasites and insects. Some antibiotics have the function of inhibiting certain specific enzymes, and some antibiotics have other biological or physiological activities. . Antibiotics are one of the most commonly used drugs today. The target antibiotic content in the sample was 74.8%, and HPLC analysis showed that it was difficult to separate impurities near its peak. The preparative column using ODS-AQ packing, the column efficiency is increased by the length of the column in series, and the separation of the target is basically achieved under isocratic conditions. The target purity of the product reached 99.4%.
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